For a consultation and quote: email or call +1-206-732-6068.

 CUSTOM SEQUENCING SOLUTIONS

Sound Genomics, a service core at the University of Washington offers next-generation sequencing and data analysis solutions.
The center is a component of the Washington National Primate Research Center and is operated by the Division of NHP Systems Biology in the Gale laboratory.

Services and Rates

We offer the following:

Illumina NextSeq 500 and Miseq
DNA/RNA library preparation and QC
Purified PCR products
Microarray processing
Bioinformatics analysis and support
RNA Isolation and QC

Custom applications REQUIRE consultation to discuss project.


Sequencing Run Costs

MiSeq RunsUW - AffiliatedNon-ProfitIndustry
v2 50 Cycle$1 273.10$1 374.95$2 392.42
v2 300 Cycle$1 509.60$1 630.37$2 836.85
v2 500 Cycle$1 636.10$1 766.99$3 074.57
v3 150 Cycle$1 361.10$1 469.99$2 557.79
v3 600 Cycle$2 059.60$2 224.37$3 870.41

Next Seq 500 RunsUW - AffiliatedNon-ProfitIndustry
Mid Output 150 Cycle$2 101.47$2 269.59$3 656.56
Mid Output 300 Cycle$2 761.47$2 982.39$4 804.96
High Output 75 Cycle$2 475.47$2 673.51$4 307.32
High Output 150 Cycle$3 833.97$4 140.69$6 671.11
High Output 300 Cycle$5 533.47$5 976.15$9 628.24

Library and QC Costs

ItemUW - AffiliatedNon-ProfitIndustry
TruSeq mRNA$186.78$201.72$351.00
TruSeq Total RNA$304.21$328.54$571.67
TruSeq Small RNA$306.54$331.07$576.05
Nextera DNA$167.84$181.26$315.40
Kapa mRNA$166.91$180.27$313.67
Kapa Total RNA$206.29$222.80$387.67
16S$126.95$137.11$238.57
Amplicon$143.23$154.69$269.16
Long Insert RNA$242.92$262.36$456.50
IsoSeq$148.81$160.71$279.64
Custom Library$402.85$435.07$757.03
Library QC$40.31$43.53$75.74
TruSeq RNA Access $382.29 $412.87 $718.40

RNA Extraction and QC Costs

ItemUW - AffiliatedNon-ProfitIndustry
Tissue to RNA$40.46$43.70$76.04
Blood to RNA$43.78$47.29$82.28
FFPE to RNA$41.89$45.24$78.72
RNA-DNA$45.48$49.12$85.46
QC$33.00$35.64$62.02

Data Analysis and Delivery

For file delivery and analysis, we offer multiple options to suit the specific needs of the end user.

An  link will be provided to download all of the raw (FASTQ) files and QC summaries.  Data can also be shared via BaseSpace. This, as well as a summary of read and run QC is provided as part of the sequencing cost.
For customers desiring a more complex and comprehensive bioinformatics analysis and processing a free consultation up to 2 hours will be provided prior to any sample processing or sequencing.  This consultation is mandatory for this type of analysis to ensure the numbersof samples and comparisons are adequate for the types of analysis desired


Analysis tier 1:

Mapping to a supplied genome/reference with or without annotation, and results in a delivery of a BAM/SAM file to the customer.  This BAM/SAM file can then be used for the next steps of data processing and analysis.


Analysis tier 2:

Targeted to users without sufficient computing resources for data processing, but which have expertise and access to bioinformatics tools for downstream functional analysis and interpretation. For RNA-seq data, raw data matrix as well as differential expression analysis will be provided to the user. For host or pathogen genetic analysis, alignments and variant files will be provided. Users will be responsible for downstream analysis.


Analysis tier 3:

Targeted to users without sufficient computing or expertise in downstream analysis and interpretation. These interactions will be more collaborative in nature. We will consult with the user to define a plan that will include any required aspects of a project, from library preparation to downstream analysis.   For RNA-seq, the user will be provided with transcript quantification and the raw data matrix as well as functional analysis based upon their specific biological questions. If necessary, we will also provide de novo assembly of transcriptome or virus genome where a specified reference is not available.


Pricing is for up to 24 samples, and tier 3 will require a custom price quote based on FTE hour units and computation units of use (CPU hours/disk storage).  There is a minimum charge of 40 hours for up to 24 samples for this tier.

Analysis Tier 1:  $2000
Analysis Tier 2:  $3000
Analysis Tier 3:  $6000 plus additional rate of $115/hr.


Requesting Sequencing Services

  1. Please contact us for a quick consultation and a quote.
  2. Submit an external service request form: SoundGenomics_Service_Request.
  3. Email the completed form to us.
  4. Prepare your samples or libraries for submission.
  5. Ship or drop of your samples to the address below.
  6. We will e-mail confirmation of receipt and what services will be performed.
  7. Await a sequencing report at the end of sequencing project, with directions on how to get data from BaseSpace if not locally delivered.

We will store locally all sequence and run data for 3 months.

 

Sample and Library Submission Guidelines

Locally libraries can be delivered to the adress below. For shipping please contact us first for guidelines and further instructions.

Shipping Samples

Sound Genomics
c/o Elise Smith Rm 252
Rosen Building
960 Republican St.
Seattle, WA 98109-4325
(206)732-6068



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